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SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse mAb

SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse mAb

     
  • 1 - SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse mAb AP94094
    Sample:
    Lane 1: SARS-CoV-2 N protein(Cat# AP94094) at 50ng
    Lane 2: SARS-CoV-2 N protein(Cat# AP94094) at 25ng
    Lane 3: SARS-CoV-2 N protein(Cat# AP94094) at 5ng
    Lane 4: SARS-CoV-2 N protein(Cat# AP94094) at 2ng
    Lane 5: SARS-CoV-2 N protein(Cat# AP94094) at 1ng
    Lane 6:SARS-CoV-2 N protein(Cat# AP94094) at 0.5ng
    Primary: Anti- SARS-CoV-2 N protein (AP94094) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 46 kD
    Observed band size: 46 kD
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Host Mouse
Clonality Monoclonal
Physical State Liquid
Immunogen Recombinant SARS-CoV-2 Nucleocapsid Protein
Epitope Specificity full length
Isotype IgG1
Purity affinity purified by Protein G
Buffer 0.01M PBS (pH7.4) with 0.02% Proclin300.
SUBCELLULAR LOCATION Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
Additional Information
Dilution WB=1:500-2000
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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