ASFV p72 Rabbit pAb

Measured by its binding ability in a indirect ELISA. Immobilized Pig ASFV p72 protein, His Tag (Cat. AP94151) at 2 µg/mL (100 µL/well) can bind Rabbit Anti-Pig ASFV p72 Antibody, the EC50 is 242.6 ng/mL.
Sample:
Lane 1: Recombinded ASFV p72 protein
Primary: Anti-ASFV p72 (AP94151) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73.1 kDa
Observed band size: 73.1 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Pig ASFV p72 protein, His Tag (Cat. bs-41384P) at 2 µg/mL (100 µL/well) can bind Rabbit Anti-Pig ASFV p72 Antibody, the EC50 is 242.6 ng/mL.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Host	Rabbit
Clonality	Polyclonal
Physical State	Liquid
Immunogen	Recombinded ASFV p72 protein
Epitope Specificity	1-646/646
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Dilution	WB=1:500-2000,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.