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Actin (Fruit Fly) Mouse mAb, Loading Control

Actin (Fruit Fly) Mouse mAb, Loading Control

     
  • 1 - Actin (Fruit Fly) Mouse mAb, Loading Control AP94209
    25 ug total protein per lane of various lysates (see on figure) probed with Actin (Fruit Fly) monoclonal antibody, unconjugated (AP94209) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Reactivity Drosophila
Host Mouse
Clonality Monoclonal
Calculated MW 42 KDa
Physical State Liquid
Immunogen Recombinant Fruit Fly Actin.
Isotype IgG1
Purity affinity purified by Protein G
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm, cytoskeleton.
SIMILARITY Belongs to the actin family.
SUBUNIT Interacts with Rab6.
Post-translational modifications Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Dilution WB=1:500-5000
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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