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PRDX2 Recombinant Mouse mAb

PRDX2 Recombinant Mouse mAb

     
  • 0 - PRDX2 Recombinant Mouse mAb AP94399
    Cell line: MCF-7
    Fixative: 4% Paraformaldehyde
    Permeabilization: 0.1% TritonX-100
    Primary ab dilution: 1:50
    Primary incubation condition: 4°C overnight
    Secondary ab: Goat Anti-Mouse IgG
    Nuclear counter stain: DAPI (Blue)
    Comment: Color green is the positive signal for AP94399
  • 1 - PRDX2 Recombinant Mouse mAb AP94399
    Sample:
    Lane 1: Mouse NIH/3T3 cell lysates
    Lane 2: Mouse Testis tissue lysates
    Lane 3: Mouse Heart tissue lysates
    Lane 4: Mouse Cerebrum tissue lysates
    Lane 5: Human Raji cell lysates
    Lane 6: Human MCF-7 cell lysates
    Lane 7: Human HeLa cell lysates
    Lane 8: Human 293T cell lysates
    Lane 9: Human MDA-MB-231 cell lysates
    Lane 10: Human HepG2 cell lysates
    Primary: Anti-PRDX2 (AP94399) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: kDa
    Observed band size: 20 kDa
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Reactivity Human, Mouse
Host Mouse
Clonality Recombinant
Physical State Liquid
Isotype IgG1, Kappa
Purity affinity purified by Protein G
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. [provided by RefSeq, Mar 2013]
Additional Information
Dilution WB=1:500-1000,ICC/IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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