TFRC Rabbit pAb

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD71 protein, His Tag (Cat. AP94411) at 2 µg/mL (100 µL/well) can bind Rabbit Anti-Mouse TFRC Antibody, the EC50 is 21.28 ng/mL.
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HT-1080 cell lysates
Primary: Anti-TFRC (AP94411) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kDa
Observed band size: 100 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD71 protein, His Tag (Cat. bs-41377P) at 2 µg/mL (100 µL/well) can bind Rabbit Anti-Mouse TFRC Antibody, the EC50 is 21.28 ng/mL.

产品详情
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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q62351
Reactivity	Human, Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	85731 Da
Physical State	Liquid
Immunogen	Recombinant Mouse CD71 protein
Epitope Specificity	89-763/763
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY	Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain.
SUBUNIT	Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC.
Post-translational modifications	N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Additional Information
Gene ID	22042
Other Names	Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, CD71, Tfrc, Trfr
Dilution	WB=1:500-2000,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Tfrc
Synonyms	Trfr
Function	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (By similarity). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Upon stimulation, positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (By similarity). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (By similarity). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (By similarity). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (PubMed:38625739).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P02786}; Single-pass type II membrane protein {ECO:0000250\|UniProtKB:P02786} Melanosome {ECO:0000250\|UniProtKB:P02786}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

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Discontinued

Cat# AP94411

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

替代产品:

TFRC Rabbit pAb

TFRC Rabbit pAb

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