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EP4 Rabbit pAb

EP4 Rabbit pAb

     
  • 0 - EP4 Rabbit pAb AP94536
    Blank control:Ctll-2.
    Primary Antibody (green line): Rabbit Anti-EP4 antibody (AP94536)
    Dilution: 2ug/Test;
    Secondary Antibody : Goat anti-rabbit IgG-FITC
    Dilution: 0.5ug/Test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • 1 - EP4 Rabbit pAb AP94536
    Sample:
    Lane 1: Mouse Uterus tissue lysates
    Lane 2: Mouse Small intestine tissue lysates
    Lane 3: Mouse Kidney tissue lysates
    Lane 4: Mouse Prostate tissue lysates
    Primary: Anti-EP4 (AP94536) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 53 kD
    Observed band size: 55 kD

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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P32240
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 56158 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from mouse EP4
Epitope Specificity 1-100/513
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cell membrane.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Additional Information
Gene ID 19219
Other Names Prostaglandin E2 receptor EP4 subtype, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, Ptger4, Ptgerep4
Dilution WB=1:500-2000,Flow-Cyt=2ug/Test
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name Ptger4
Synonyms Ptgerep4
Function Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus
Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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