LDHA Recombinant Rabbit mAb

25 ug total protein per lane of various lysates (see on figure) probed with LDHA monoclonal antibody, unconjugated (AP94838) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LDHA Monoclonal Antibody, Unconjugated(AP94838) at 1:200 overnight at 4°C, followed by conjugation to the AP94838-HRP and DAB (C-0010) staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, IP
Primary Accession	P00338
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Recombinant
Calculated MW	36689 Da
Physical State	Liquid
Immunogen	A synthesized peptide derived from human LDHA
Epitope Specificity	254-300
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm.
DISEASE	Glycogen storage disease 11 (GSD11) [MIM:612933]: A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. Note=The disease is caused by mutations affecting the gene represented in this entry.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD+.

Additional Information
Gene ID	3939
Other Names	L-lactate dehydrogenase A chain, LDH-A, 1.1.1.27, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA (HGNC:6535)
Dilution	WB=1:1000-5000,IHC-P=1:200-1000,IHC-F=1:200-1000,ICC/IF=1:100-500,IF=1:200-1000,IP=1:50-100,Flow-Cyt=1:100-200
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	LDHA (HGNC:6535)
Function	Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Cellular Location	Cytoplasm.
Tissue Location	Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.