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PCYT1A Rabbit pAb

PCYT1A Rabbit pAb

     
  • 14 - PCYT1A Rabbit pAb AP94840
    Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (AP94840) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - PCYT1A Rabbit pAb AP94840
    Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (AP94840) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - PCYT1A Rabbit pAb AP94840
    Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (AP94840) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - PCYT1A Rabbit pAb AP94840
    Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (AP94840) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - PCYT1A Rabbit pAb AP94840
    Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (AP94840) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF
Primary Accession P49585
Reactivity Rat, Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 41731 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human PCYT1A
Epitope Specificity 201-300/367
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm; cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
SIMILARITY Belongs to the cytidylyltransferase family.
SUBUNIT Homodimer.
Post-translational modifications The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Gene ID 5130
Other Names Choline-phosphate cytidylyltransferase A, 2.7.7.15, CCT-alpha, CTP:phosphocholine cytidylyltransferase A, CCT A, CT A, Phosphorylcholine transferase A, PCYT1A, CTPCT, PCYT1
Target/Specificity Cancer,Neurotrophins,Lipid metabolism
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name PCYT1A
Synonyms CTPCT, PCYT1
Function Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:P19836}. Membrane {ECO:0000250|UniProtKB:P19836}; Peripheral membrane protein {ECO:0000250|UniProtKB:P19836}. Endoplasmic reticulum membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P19836}. Nucleus Note=It can interconvert between an inactive cytosolic form and an active membrane-bound form. {ECO:0000250|UniProtKB:P19836}
Tissue Location Brain, placenta, liver, fetal and adult lung.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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