Recombinant Anti-GLUT1 Rabbit mAb

Western blot analysis of GLUT1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD)
Immunohistochemical analysis of GLUT1 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P11166
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	54084 Da

Additional Information
Gene ID	6513
Other Names	GLUT1; Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter
Dilution	WB~~1:500 - 1:1000 IHC~~1:100~500
Format	The antibody was purified by immunogen affinity chromatography. iquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	SLC2A1 (HGNC:11005)
Function	Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:10227690, PubMed:10954735, PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy- independent, facilitative transport of glucose into the brain (PubMed:10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Melanosome. Photoreceptor inner segment {ECO:0000250\|UniProtKB:P17809}. Note=Localizes primarily at the cell surface (PubMed:18245775, PubMed:19449892, PubMed:23219802, PubMed:24847886, PubMed:25982116). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065)
Tissue Location	Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues