Recombinant Anti-Alpha-tubulin (Acetyl-K40) Rabbit mAb

Western blot analysis of Alpha-tubulin (Acetyl-K40) expression in HT1080 (A) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)
Immunofluorescent analysis of Alpha-tubulin (Acetyl-K40) staining in HT1080 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IP, IF/IC
Primary Accession	Q71U36
Other Accession	P68363, P68366
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	50136 Da

Additional Information
Gene ID	7846
Other Names	TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain
Target/Specificity	KLH-conjugated synthetic Acetyl-peptide corresponding to residues surrounding K40 of human Alpha-tubulin. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IP~~N/A IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TUBA1A
Synonyms	TUBA3
Function	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location	Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000250\|UniProtKB:P68369}
Tissue Location	Expressed at a high level in fetal brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.