Recombinant Anti-MMP8 Rabbit mAb

Western blot analysis of MMP8 expression in human placenta (A) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 70 kD)
Immunofluorescent analysis of MMP8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IP, IF/IC
Primary Accession	P22894
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	53412 Da

Additional Information
Gene ID	4317
Other Names	CLG1; Neutrophil collagenase; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human MMP8 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IP~~N/A IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MMP8
Synonyms	CLG1
Function	Can degrade fibrillar type I, II, and III collagens.
Cellular Location	Cytoplasmic granule. Secreted, extracellular space, extracellular matrix. Note=Stored in intracellular granules
Tissue Location	Neutrophils.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.