Recombinant Anti-Gamma-catenin Rabbit mAb

Western blot analysis of Gamma-catenin expression in A431 (A) whole cell lysates. (Predicted band size: 82 kD; Observed band size: 82 kD)
Immunofluorescent analysis of Gamma-catenin staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	P14923
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	81745 Da

Additional Information
Gene ID	3728
Other Names	CTNNG; DP3; Junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human Gamma-catenin protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	JUP (HGNC:6207)
Function	Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E- cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).
Cellular Location	Cell junction, adherens junction. Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Cytoplasm {ECO:0000250\|UniProtKB:Q9PVF7}. Cell junction {ECO:0000250\|UniProtKB:Q9PVF7}. Nucleus {ECO:0000250\|UniProtKB:Q9PVF7} Cell projection, axon {ECO:0000250\|UniProtKB:Q02257}. Note=Cytoplasmic in a soluble and membrane-associated form. Colocalizes with DSG4 at desmosomes (PubMed:21495994).
Tissue Location	Expressed in cardiomyocytes in the heart (at protein level). Expressed by keratinocytes in the skin (at protein level) (PubMed:26604139).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.