Recombinant Anti-GHR Rabbit mAb

Western blot analysis of GHR expression in MCF7 (A) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 140 kD)
Immunofluorescent analysis of GHR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	P10912
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	71500 Da

Additional Information
Gene ID	2690
Other Names	Growth hormone receptor; GH receptor; Somatotropin receptor
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human GHR protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GHR
Function	Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth (PubMed:1549776, PubMed:2825030, PubMed:8943276). On ligand binding, couples to the JAK2/STAT5 pathway (PubMed:1549776, PubMed:15690087, PubMed:2825030, PubMed:8943276).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway {ECO:0000250\|UniProtKB:P19941} [Growth hormone-binding protein]: Secreted. Note=Complexed to a substantial fraction of circulating GH.
Tissue Location	Expressed in various tissues with high expression in liver and skeletal muscle. [Isoform 4]: Predominantly expressed in kidney, bladder, adrenal gland and brain stem (PubMed:1569971). Highly expressed in placental villi (PubMed:1569971, PubMed:8360189)