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Recombinant Anti-GABARAP Rabbit mAb

Western blot analysis of GABARAP expression in HepG2 (A) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 17 kD)
Immunofluorescent analysis of GABARAP staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	O95166
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	13918 Da

Additional Information
Gene ID	11337
Other Names	FLC3B; Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human GABARAP protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GABARAP (HGNC:4067)
Synonyms	FLC3B
Function	Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). Participates in selective ER-phagy through interaction with UBAC2, promoting recruitment of UBAC2-associated ER fragments to autophagosomes (PubMed:39284914).
Cellular Location	Cytoplasmic vesicle, autophagosome membrane. Endomembrane system {ECO:0000250\|UniProtKB:P60517}. Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:P60517}. Golgi apparatus membrane {ECO:0000250\|UniProtKB:P60517}. Cytoplasmic vesicle {ECO:0000250\|UniProtKB:P60517}. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity) Also localizes to discrete punctae along the ciliary axoneme (By similarity). {ECO:0000250\|UniProtKB:P60517, ECO:0000250\|UniProtKB:Q9DCD6}
Tissue Location	Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.