Recombinant Anti-OCT2 Rabbit mAb

Western blot analysis of OCT2 expression in Ramos (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 58 kD)
Immunofluorescent analysis of OCT2 staining in Ramos cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IP, IF/IC
Primary Accession	P09086
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	51209 Da

Additional Information
Gene ID	5452
Other Names	OCT2; OTF2; POU domain, class 2, transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human OCT2 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) FC~~1:10~50 IP~~N/A IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	POU2F2 (HGNC:9213)
Synonyms	OCT2, OTF2
Function	Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874).
Cellular Location	Cytoplasm. Nucleus.
Tissue Location	Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.