Recombinant Anti-Serpin A8 Rabbit mAb

Western blot analysis of Serpin A8 expression in HepG2 (A) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 53-64 kD)
Immunofluorescent analysis of Serpin A8 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IP, IF/IC
Primary Accession	P01019
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Calculated MW	52070 Da

Additional Information
Gene ID	183
Other Names	SERPINA8; Angiotensinogen; Serpin A8
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human Serpin A8 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) FC~~1:10~50 IP~~N/A IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	AGT (HGNC:333)
Synonyms	SERPINA8
Function	Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
Cellular Location	Secreted
Tissue Location	Expressed by the liver and secreted in plasma.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.