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Recombinant Anti-IKK epsilon Rabbit mAb

Western blot analysis of IKK epsilon expression in Raji (A) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 80 kD)
Immunofluorescent analysis of IKK epsilon staining in Raji cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	Q14164
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Calculated MW	80462 Da

Additional Information
Gene ID	9641
Other Names	IKKE; IKKI; KIAA0151; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; Inducible I kappa-B kinase; IKK-i
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human IKK epsilon protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IKBKE
Synonyms	IKKE, IKKI, KIAA0151
Function	Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling (PubMed:16840782, PubMed:18583960, PubMed:19153231, PubMed:19481526, PubMed:21138416, PubMed:22532683, PubMed:23453969, PubMed:23478265, PubMed:24882218, PubMed:32268090). Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling (PubMed:18583960, PubMed:19153231, PubMed:22532683, PubMed:23478265). Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X (PubMed:18583960, PubMed:19153231, PubMed:22532683, PubMed:23478265). This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB (PubMed:18583960, PubMed:19153231, PubMed:22532683, PubMed:23478265). In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli (PubMed:17568778). Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes (PubMed:17568778). In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708' (By similarity). Phosphorylation of STAT1 at 'Ser-708' also seems to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses (By similarity). Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway via the phosphorylation of CYLD, NFKBIA and p65/RELA (PubMed:16840782, PubMed:19481526, PubMed:21138416, PubMed:23453969). Activates NF-kappa- B by mediating phosphorylation and inactivation of CYLD (PubMed:19481526). Phosphorylates inhibitors of NF-kappa-B, such as NFKBIA, thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor (PubMed:10882136). Also activates NF-kappa-B signaling by mediating phosphorylation and activation of STING1 (PubMed:32268090, PubMed:39262777, PubMed:40973797). Protects cells against DNA damage- induced cell death (PubMed:20188669). Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, which leads to a negative impact on insulin sensitivity (By similarity). Phosphorylates AKT1 (PubMed:21464307).
Cellular Location	Cytoplasm. Nucleus. Nucleus, PML body. Note=Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent (PubMed:20188669) Located diffusely throughout the cytoplasm but locates to punctate cytoplasmic bodies when coexpressed with TRIM6 (PubMed:24882218)
Tissue Location	Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon