Recombinant Anti-Monoamine Oxidase A Rabbit mAb

Western blot analysis of Monoamine Oxidase A expression in HepG2 (A) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 60 kD)
Immunofluorescent analysis of Monoamine Oxidase A staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	P21397
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	59682 Da

Additional Information
Gene ID	4128
Other Names	Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human Monoamine Oxidase A protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MAOA (HGNC:6833)
Function	Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed:18391214, PubMed:20493079, PubMed:24169519, PubMed:8316221). Preferentially oxidizes serotonin (PubMed:20493079, PubMed:24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).
Cellular Location	Mitochondrion outer membrane {ECO:0000250\|UniProtKB:P21396}; Single-pass type IV membrane protein {ECO:0000250\|UniProtKB:P21396}; Cytoplasmic side {ECO:0000250\|UniProtKB:P21396}
Tissue Location	Heart, liver, duodenum, blood vessels and kidney.