Recombinant Anti-Ribophorin-1 Rabbit mAb

Western blot analysis of Ribophorin-1 expression in Jurkat (A) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 69 kD)
Immunofluorescent analysis of Ribophorin-1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	P04843
Reactivity	Human, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	68569 Da

Additional Information
Gene ID	6184
Other Names	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Ribophorin I; RPN-I; Ribophorin-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human Ribophorin-1 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RPN1 (HGNC:10381)
Function	Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity).
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:E2RQ08}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:E2RQ08}. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Location	Expressed in all tissues tested.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.