Recombinant Anti-KLC3 Rabbit mAb

Western blot analysis of KLC3 expression in Jurkat (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)
Immunofluorescent analysis of KLC3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IP, IF/IC
Primary Accession	Q6P597
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Calculated MW	55364 Da

Additional Information
Gene ID	147700
Other Names	KLC2; KLC2L; Kinesin light chain 3; KLC2-like; kinesin light chain 2
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human KLC3 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IP~~N/A IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KLC3
Synonyms	KLC2, KLC2L
Function	Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa (By similarity). May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece (By similarity).
Cellular Location	Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:Q68G30, ECO:0000250\|UniProtKB:Q91W40} Mitochondrion {ECO:0000250\|UniProtKB:Q91W40}. Note=In elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria. Also localizes to the manchette in elongating spermatids. {ECO:0000250\|UniProtKB:Q68G30, ECO:0000250\|UniProtKB:Q91W40}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.