Recombinant Anti-AIOLOS Rabbit mAb

Western blot analysis of AIOLOS expression in Ramos (A) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 70 kD)
Immunofluorescent analysis of AIOLOS staining in Ramos cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	Q9UKT9
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Calculated MW	58023 Da

Additional Information
Gene ID	22806
Other Names	ZNFN1A3; Zinc finger protein Aiolos; Ikaros family zinc finger protein 3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human AIOLOS protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IKZF3
Synonyms	ZNFN1A3
Function	Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Cellular Location	Nucleus. Cytoplasm [Isoform 3]: Nucleus [Isoform 14]: Nucleus. Cytoplasm
Tissue Location	Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.