Recombinant Anti-Histone H3 (MonoMethyl-K14) Rabbit mAb

Western blot analysis of Histone H3 (MonoMethyl-K14) expression in Jurkat (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)
Immunofluorescent analysis of Histone H3 (MonoMethyl-K14) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	P68431
Other Accession	Q71DI3, P84243, Q6NXT2
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Calculated MW	15404 Da

Additional Information
Gene ID	8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Other Names	H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Target/Specificity	KLH-conjugated synthetic MonoMethyl-peptide corresponding to residues surrounding K14 of human Histone H3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) FC~~1:10~50 IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	H3C1 (HGNC:4766)
Synonyms	H3FA, HIST1H3A
Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location	Nucleus. Chromosome.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.