Recombinant Anti-NDUFAF1 Rabbit mAb

Western blot analysis of NDUFAF1 expression in HEK293T (A) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 33 kD)
Immunofluorescent analysis of NDUFAF1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IP, IF/IC
Primary Accession	Q9Y375
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Calculated MW	37764 Da

Additional Information
Gene ID	51103
Other Names	CIA30; Complex I intermediate-associated protein 30, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within human NDUFAF1 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~1:10~50 IP~~N/A IF/IC~~N/A
Format	Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NDUFAF1 (HGNC:18828)
Synonyms	CIA30
Function	As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Cellular Location	Mitochondrion. Mitochondrion matrix. Note=Peripherally associated with the matrix face of the mitochondrial inner membrane
Tissue Location	Ubiquitous..

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.