Anti-ABCG5 Antibody

Western blot analysis of ABCG5 expression in HL7702 (A), Raji (B), Jurkat (C) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 72 kD)
Immunohistochemical analysis of ABCG5 staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of A549 cells using Anti-ABCG5 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	Q9H222
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	72504 Da

Additional Information
Gene ID	64240
Other Names	ATP-binding cassette sub-family G member 5; Sterolin-1
Target/Specificity	Recombinant fusion protein of human ABCG5 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ABCG5 (HGNC:13886)
Function	ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane (PubMed:27144356). Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile (PubMed:11099417, PubMed:11138003, PubMed:15054092, PubMed:27144356). Required for normal sterol homeostasis (PubMed:11099417, PubMed:11138003, PubMed:15054092). The heterodimer with ABCG8 has ATPase activity (PubMed:16893193, PubMed:20210363, PubMed:27144356).
Cellular Location	Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein
Tissue Location	Strongly expressed in the liver, lower levels in the small intestine and colon.