Anti-Acetylcholinesterase Antibody

Western blot analysis of Acetylcholinesterase expression in PC12 (A), Hela (B), mouse brain (C), NIH/3T3 (D), COS7 (E), Jurkat (F), Raji (G) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 72 kD)
Immunohistochemical analysis of Acetylcholinesterase staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of NIH/3T3 cells using Anti-Acetylcholinesterase Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P22303
Reactivity	Human, Mouse, Rat, Mk
Host	Mouse
Clonality	Monoclonal
Calculated MW	67796 Da

Additional Information
Gene ID	43
Other Names	Acetylcholinesterase; AChE
Target/Specificity	Recombinant fusion protein of human Acetylcholinesterase expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACHE (HGNC:108)
Function	Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Cellular Location	Synapse. Secreted. Cell membrane; Peripheral membrane protein [Isoform H]: Cell membrane; Lipid- anchor, GPI-anchor; Extracellular side
Tissue Location	Isoform H is highly expressed in erythrocytes.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.