Anti-AEBP2 Antibody

Western blot analysis of AEBP2 expression in COS7 (A), HepG2 (B), SKMES1 (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 54 kD)
Immunofluorescent analysis of AEBP2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of MCF7 cells using Anti-AEBP2 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	Q6ZN18
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	54467 Da

Additional Information
Gene ID	121536
Other Names	Zinc finger protein AEBP2; Adipocyte enhancer-binding protein 2; AE-binding protein 2
Target/Specificity	Recombinant fusion protein of human AEBP2 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	AEBP2
Function	Acts as an accessory subunit for the core Polycomb repressive complex 2 (PRC2), which mediates histone H3K27 (H3K27me3) trimethylation on chromatin leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:29499137, PubMed:31959557). Plays a role in nucleosome localization of the PRC2 complex (PubMed:29499137).
Cellular Location	Nucleus. Note=Localizes to chromatin as part of the PRC2 complex

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.