Anti-ALDOA Antibody

Western blot analysis of ALDOA expression in MCF7 (A), Hela (B), NIH/3T3 (C) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 39 kD)
Immunohistochemical analysis of ALDOA staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of K562 cells using Anti-ALDOA Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P04075
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Calculated MW	39420 Da

Additional Information
Gene ID	226
Other Names	ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase
Target/Specificity	Recombinant fusion protein of human ALDOA expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ALDOA (HGNC:414)
Synonyms	ALDA
Function	Catalyzes the reversible conversion of beta-D-fructose 1,6- bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, also functions as a scaffolding protein (By similarity). In response to glucose deprivation, FBP dissociates from aldolase and acts as an adapter that promotes AMP-activated protein kinase (AMPK) activity: mechanistically, associates with transient receptor potential channels TrpV (TRPV1-TRPV4), promoting inhibition of the V-ATPase complex on lysosomes and AMPK activation via the AXIN1-STK11/LKB1 axis (By similarity).
Cellular Location	Cytoplasm, myofibril, sarcomere, I band {ECO:0000250\|UniProtKB:P00883}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000250\|UniProtKB:P00883}. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+) {ECO:0000250\|UniProtKB:P00883}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.