Anti-CD40 Antibody

Western blot analysis of CD40 expression in Raji (A) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 43 kD)
Immunohistochemical analysis of CD40 staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of Raji cells using Anti-CD40 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P25942
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	30619 Da

Additional Information
Gene ID	958
Other Names	TNFRSF5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40
Target/Specificity	Recombinant fusion protein of human CD40 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CD40
Synonyms	TNFRSF5
Function	Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Cellular Location	[Isoform I]: Cell membrane; Single-pass type I membrane protein
Tissue Location	B-cells and in primary carcinomas.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.