Anti-CD99 Antibody

Western blot analysis of CD99 expression in THP1 (A), U937 (B), MOLT4 (C), L1210 (D) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 32 kD)
Immunohistochemical analysis of CD99 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of Jurkat cells using Anti-CD99 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P14209
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Calculated MW	18848 Da

Additional Information
Gene ID	4267
Other Names	MIC, 2; MIC, 2X; MIC, 2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC, 2; T-cell surface glycoprotein E2; CD99
Target/Specificity	Recombinant fusion protein of human CD99 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CD99
Synonyms	MIC2, MIC2X, MIC2Y
Function	Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Cellular Location	Membrane; Single-pass type I membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.