Anti-Collagen 2 alpha 1 Antibody

Western blot analysis of Collagen 2 alpha 1 expression in Hela (A), MCF7 (B), A549 (C), Jurkat (D), K562 (E) whole cell lysates. (Predicted band size: 142 kD; Observed band size: 130 kD)
Immunofluorescent analysis of Collagen 2 alpha 1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Jurkat cells using Anti-Collagen 2 alpha 1 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	P02458
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	141785 Da

Additional Information
Gene ID	1280
Other Names	Collagen alpha-1(II) chain; Alpha-1 type II collagen
Target/Specificity	Recombinant fusion protein of human Collagen 2 alpha 1 expressed in HEK293
Dilution	WB~~WB (1/500 - 1/1000) FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	COL2A1 (HGNC:2200)
Function	Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Cellular Location	Secreted, extracellular space, extracellular matrix {ECO:0000255\|PROSITE-ProRule:PRU00793}
Tissue Location	Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.