Anti-CD131 Antibody

Western blot analysis of CD131 expression in NIH/3T3 (A), Hela (B), C6 (C), A549 (D), MCF7 (E) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 97 kD)
Immunohistochemical analysis of CD131 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-CD131 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P32927
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	97336 Da

Additional Information
Gene ID	1439
Other Names	IL3RB; IL5RB; Cytokine receptor common subunit beta; CDw131; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131
Target/Specificity	Recombinant fusion protein of human CD131 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CSF2RB
Synonyms	IL3RB, IL5RB
Function	Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA (PubMed:1495999). In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK- STAT pathway (PubMed:9516124).
Cellular Location	Membrane; Single-pass type I membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.