Anti-Cytochrome c Antibody

Western blot analysis of Cytochrome c expression in Hela (A), Jurkat (B), RAW264.7 (C), NIH/3T3 (D), HL60 (E), K562 (F), HepG2 (G), MCF7 (H) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 12 kD)
Immunohistochemical analysis of Cytochrome c staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-Cytochrome c Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P99999
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Calculated MW	11749 Da

Additional Information
Gene ID	54205
Other Names	CYC; Cytochrome c
Target/Specificity	Recombinant fusion protein of human Cytochrome c expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CYCS
Synonyms	CYC
Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Cellular Location	Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.