Anti-DCTN4 Antibody

Western blot analysis of DCTN4 expression in Raw264.7 (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 54 kD)
Immunohistochemical analysis of DCTN4 staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of DCTN4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of A549 cells using Anti-DCTN4 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	Q9UJW0
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	52337 Da

Additional Information
Gene ID	51164
Other Names	Dynactin subunit 4; Dyn4; Dynactin subunit p62
Target/Specificity	Recombinant fusion protein of human DCTN4 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DCTN4 (HGNC:15518)
Function	Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.
Cellular Location	Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250\|UniProtKB:Q9QUR2}. Cytoplasm, cell cortex {ECO:0000250\|UniProtKB:Q9QUR2}. Cytoplasm, myofibril, sarcomere {ECO:0000250\|UniProtKB:Q8CBY8}. Note=Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (PubMed:10671518). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex (By similarity). In skeletal muscles, costamere localization requires the presence of ANK2 (By similarity) {ECO:0000250\|UniProtKB:Q8CBY8, ECO:0000250\|UniProtKB:Q9QUR2, ECO:0000269\|PubMed:10671518}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.