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Anti-PSD95 Antibody

Western blot analysis of PSD95 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 95 kD)
Immunohistochemical analysis of PSD95 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of LNCAP cells using Anti-PSD95 Antibody (green) and negative control (red).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P78352
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	80495 Da

Additional Information
Gene ID	1742
Other Names	PSD95; Disks large homolog 4; PostsynaptIC, density protein 95; PSD-95; Synapse-associated protein 90; SAP-90; SAP90
Target/Specificity	Recombinant fusion protein of human PSD95 expressed in HEK293
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DLG4 (HGNC:2903)
Synonyms	PSD95
Function	Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (PubMed:26334723).
Cellular Location	Cell membrane; Lipid-anchor; Cytoplasmic side. Postsynaptic density {ECO:0000250\|UniProtKB:P31016}. Synapse Cytoplasm {ECO:0000250\|UniProtKB:P31016}. Cell projection, axon {ECO:0000250\|UniProtKB:P31016}. Cell projection, dendritic spine {ECO:0000250\|UniProtKB:P31016}. Cell projection, dendrite {ECO:0000250\|UniProtKB:P31016}. Presynapse {ECO:0000250\|UniProtKB:P31016}. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. Suppression of neuronal activity induces synaptic accumulation and clustering of DLG4. {ECO:0000250\|UniProtKB:P31016}
Tissue Location	Brain.