Anti-Ferritin L Antibody

Western blot analysis of Ferritin L expression in HepG2 (A), K562 (B) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 22 kD)
Immunohistochemical analysis of Ferritin L staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HepG2 cells using Anti-Ferritin L Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P02792
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	20020 Da

Additional Information
Gene ID	2512
Other Names	Ferritin light chain; Ferritin L subunit
Target/Specificity	Recombinant fusion protein of human Ferritin L expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FTL
Function	Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
Cellular Location	Cytoplasmic vesicle, autophagosome. Cytoplasm {ECO:0000250\|UniProtKB:P29391}. Autolysosome {ECO:0000250\|UniProtKB:P29391}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.