Anti-CD42a Antibody

Western blot analysis of CD42a expression in PANC1 (A), L1210 (B) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 32 kD)
Immunohistochemical analysis of CD42a staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of K562 cells using Anti-CD42a Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P14770
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Calculated MW	19046 Da

Additional Information
Gene ID	2815
Other Names	Platelet glycoprotein IX; GP-IX; GPIX; Glycoprotein 9; CD42a
Target/Specificity	Recombinant fusion protein of human CD42a expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GP9
Function	The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
Cellular Location	Membrane; Single-pass type I membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.