Anti-IDH1 Antibody

Western blot analysis of IDH1 expression in HepG2 (A), NIH/3T3 (B), C2C12 (C), COS7 (D), SW480 (E) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 49 kD)
Immunofluorescent analysis of IDH1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-IDH1 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	O75874
Reactivity	Human, Mouse, Mk
Host	Mouse
Clonality	Monoclonal
Calculated MW	46659 Da

Additional Information
Gene ID	3417
Other Names	PIC, D; Isocitrate dehydrogenase [NADP] cytoplasmIC, ; IDH; CytosolIC, NADP-isocitrate dehydrogenase; IDP; NADP(+)-specifIC, IC, DH; Oxalosuccinate decarboxylase
Target/Specificity	Recombinant fusion protein of human IDH1 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IDH1
Synonyms	PICD
Function	Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Cellular Location	Cytoplasm, cytosol. Peroxisome

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.