Anti-SLM-1 Antibody

Western blot analysis of SLM-1 expression in K562 (A), HEK293 (B), NTERA2 (C), Hela (D), HepG2 (E), Jurkat (F), A431 (G), NIH/3T3 (H) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 39 kD)
Immunohistochemical analysis of SLM-1 staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of K562 cells using Anti-SLM-1 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	Q5VWX1
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Calculated MW	38927 Da

Additional Information
Gene ID	202559
Other Names	SLM1; KH domain-containing, RNA-binding, signal transduction-associated protein 2; Sam68-like mammalian protein 1; SLM-1; hSLM-1
Target/Specificity	Recombinant fusion protein of human SLM-1 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KHDRBS2
Synonyms	SLM1
Function	RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability (By similarity). Induces an increased concentration-dependent incorporation of exon in CD44 pre- mRNA by direct binding to purine-rich exonic enhancer. Can regulate alternative splicing of NRXN1 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. Regulates cell-type specific alternative splicing of NRXN1 at AS4 and acts synergystically with SAM68 in exon skipping. In contrast acts antagonistically with SAM68 in NRXN3 exon skipping at AS4. Its phosphorylation by FYN inhibits its ability to regulate splice site selection. May function as an adapter protein for Src kinases during mitosis.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:Q9WU01}.
Tissue Location	Highly expressed in brain, lung, kidney and small intestine. Weakly expressed in placenta, liver, spleen, thymus, ovary and colon.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.