Anti-Cytokeratin 10 Antibody

Western blot analysis of Cytokeratin 10 expression in A431 (A), C6 (B), COS7 (C), Jurkat (D), NIH/3T3 (E), HEK293 (F) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 59 kD)
Immunohistochemical analysis of Cytokeratin 10 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-Cytokeratin 10 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P13645
Reactivity	Human, Mouse, Rat, Mk
Host	Mouse
Clonality	Monoclonal
Calculated MW	58827 Da

Additional Information
Gene ID	3858
Other Names	KPP; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10
Target/Specificity	Recombinant fusion protein of human Cytokeratin 10 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KRT10 (HGNC:6413)
Synonyms	KPP
Function	Structural component of intermediate filaments in suprabasal keratinocytes of stratified epithelia. Forms heteropolymers primarily with the type II keratin KRT1, and also with KRT2, assembling into intermediate filament networks that support terminal differentiation, mechanical resilience, and epidermal barrier formation.
Cellular Location	Secreted, extracellular space. Cell surface. Cytoplasm
Tissue Location	Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines (PubMed:19627498). Localized on the surface of desquamated nasal epithelial cells (at protein level) (PubMed:12427098)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.