Anti-Cytokeratin 15 Antibody

Western blot analysis of Cytokeratin 15 expression in A431 (A), Hela (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 49 kD)
Immunohistochemical analysis of Cytokeratin 15 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of PC3 cells using Anti-Cytokeratin 15 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P19012
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	49212 Da

Additional Information
Gene ID	3866
Other Names	KRTB; Keratin, type I cytoskeletal 15; Cytokeratin-15; CK-15; Keratin-15; K15
Target/Specificity	Recombinant fusion protein of human Cytokeratin 15 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KRT15
Synonyms	KRTB
Tissue Location	Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level) Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.