Anti-MKK5 Antibody

Western blot analysis of MKK5 expression in Jurkat (A), A431 (B), A549 (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50 kD)
Immunohistochemical analysis of MKK5 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of Jurkat cells using Anti-MKK5 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	Q13163
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	50112 Da

Additional Information
Gene ID	5607
Other Names	MEK5; MKK5; PRKMK5; Dual specifIC, ity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5
Target/Specificity	Recombinant fusion protein of human MKK5 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MAP2K5
Synonyms	MEK5, MKK5, PRKMK5
Function	Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Tissue Location	Expressed in many adult tissues. Abundant in heart and skeletal muscle

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.