Anti-MEF2C Antibody

Western blot analysis of MEF2C expression in NIH3T3 (A), 3T3L1 (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 50 kD)
Immunohistochemical analysis of MEF2C staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-MEF2C Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	Q06413
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Calculated MW	51221 Da

Additional Information
Gene ID	4208
Other Names	Myocyte-specifIC, enhancer factor 2C
Target/Specificity	Recombinant fusion protein of human MEF2C expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MEF2C (HGNC:6996)
Function	Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:A0A096MJY4}. Cytoplasm, sarcoplasm {ECO:0000250\|UniProtKB:A0A096MJY4}
Tissue Location	Expressed in brain and skeletal muscle.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.