Anti-GDF8 Antibody

Western blot analysis of GDF8 expression in LNcap (A) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 39 kD)
Immunohistochemical analysis of GDF8 staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of LNCAP cells using Anti-GDF8 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	O14793
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	42750 Da

Additional Information
Gene ID	2660
Other Names	GDF8; Growth/differentiation factor 8; GDF-8; Myostatin
Target/Specificity	Recombinant fusion protein of human GDF8 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MSTN
Synonyms	GDF8
Function	Acts specifically as a negative regulator of skeletal muscle growth.
Cellular Location	Secreted {ECO:0000250\|UniProtKB:O08689}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.