Anti-Metadherin Antibody

Western blot analysis of Metadherin expression in MCF7 (A), T47D (B), Jurkat (C), K562 (D), Hela (E), PC3 (F), HepG2 (G), A431 (H), COS7 (I) whole cell lysates. (Predicted band size: 75 kD; Observed band size: 75 kD)
Immunohistochemical analysis of Metadherin staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Metadherin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-Metadherin Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	Q86UE4
Reactivity	Human, Mk
Host	Mouse
Clonality	Monoclonal
Calculated MW	63837 Da

Additional Information
Gene ID	92140
Other Names	AEG1; LYRIC, ; Protein LYRIC, ; 3D3/LYRIC, ; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rIC, h CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein
Target/Specificity	Recombinant fusion protein of human Metadherin expressed in HEK293
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MTDH
Synonyms	AEG1, LYRIC
Function	Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
Cellular Location	Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction Nucleus, nucleolus. Cytoplasm, perinuclear region Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF causes translocation from the cytoplasm to the nucleus.
Tissue Location	Widely expressed with highest levels in muscle- dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.