Anti-MUC13 Antibody

Western blot analysis of MUC13 expression in HT19 (A), SW620 (B), NIH/3T3 (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
Immunohistochemical analysis of MUC13 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of RSC96 cells using Anti-MUC13 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	Q9H3R2
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	54604 Da

Additional Information
Gene ID	56667
Other Names	DRCC1; RECC; Mucin-13; MUC-13; Down-regulated in colon cancer 1
Target/Specificity	Recombinant fusion protein of human MUC13 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MUC13
Synonyms	DRCC1, RECC
Function	Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
Cellular Location	Cell membrane; Single-pass type I membrane protein Apical cell membrane. Secreted. Note=Also exists as a soluble form
Tissue Location	Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.