Anti-CD273 Antibody

Western blot analysis of CD273 expression in HepG2 (A), Hela (B), NIH/3T3 (C), PC12 (D), K562 (E), Jurkat (F), MCF7 (G), HEK293-6e (H) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 58 kD)
Immunofluorescent analysis of CD273 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-CD273 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	Q9BQ51
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	30957 Da

Additional Information
Gene ID	80380
Other Names	B7DC; CD273; PDCD1L2; PDL2; Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273
Target/Specificity	Recombinant fusion protein of human CD273 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PDCD1LG2
Synonyms	B7DC, CD273, PDCD1L2, PDL2
Function	Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11224527, PubMed:12538684). Acts as a ligand for the inhibitory receptor PDCD1/PD-1, inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production (PubMed:11224527).
Cellular Location	[Isoform 3]: Secreted [Isoform 1]: Cell membrane; Single-pass type I membrane protein
Tissue Location	Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus