Anti-RRM1 Antibody

Western blot analysis of RRM1 expression in Hela (A), Jurkat (B), A431 (C) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 85 kD)
Immunohistochemical analysis of RRM1 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-RRM1 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P23921
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	90070 Da

Additional Information
Gene ID	6240
Other Names	RR1; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit
Target/Specificity	Recombinant fusion protein of human RRM1 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RRM1
Synonyms	RR1
Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Cellular Location	Cytoplasm.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.