Anti-SOD2 Antibody

Western blot analysis of SOD2 expression in Hela (A), HepG2 (B), SHSY5Y (C) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 23 kD)
Immunohistochemical analysis of SOD2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of MCF7 cells using Anti-SOD2 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P04179
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	24750 Da

Additional Information
Gene ID	6648
Other Names	Superoxide dismutase [Mn], mitochondrial
Target/Specificity	Recombinant fusion protein of human SOD2 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SOD2
Function	Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Cellular Location	Mitochondrion matrix.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.