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Anti-CD43 Antibody

Western blot analysis of CD43 expression in HEK293-6E (A), HEK293 (B), K562 (C), MOLT4 (D), Jurkat (E), PANC1 (F) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 50 kD)
Immunohistochemical analysis of CD43 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of Jurkat cells using Anti-CD43 Antibody (green) and negative control (red).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P16150
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	40322 Da

Additional Information
Gene ID	6693
Other Names	CD43; Leukosialin; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD43
Target/Specificity	Recombinant fusion protein of human CD43 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SPN
Synonyms	CD43
Function	Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E- selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).
Cellular Location	Membrane; Single-pass type I membrane protein. Cell projection, microvillus {ECO:0000250\|UniProtKB:P13838}. Cell projection, uropodium {ECO:0000250\|UniProtKB:P15702}. Note=Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN) {ECO:0000250\|UniProtKB:P13838, ECO:0000250\|UniProtKB:P15702}
Tissue Location	Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas