Anti-Synapsin 1 Antibody

Western blot analysis of Synapsin 1 expression in SKNSH (A), NIH/3T3 (B), U251 (C), C6 (D), A549 (E), MCF7 (F), COS7 (G) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 74 kD)
Immunohistochemical analysis of Synapsin 1 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Synapsin 1 staining in GC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-Synapsin 1 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IF/IC
Primary Accession	P17600
Reactivity	Human, Mouse, Rat, Mk
Host	Mouse
Clonality	Monoclonal
Calculated MW	74111 Da

Additional Information
Gene ID	6853
Other Names	Synapsin-1; Brain protein 4.1; Synapsin I
Target/Specificity	Recombinant fusion protein of human Synapsin 1 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SYN1
Function	Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).
Cellular Location	Synapse {ECO:0000250\|UniProtKB:O88935}. Golgi apparatus {ECO:0000250\|UniProtKB:O88935}. Presynapse. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle {ECO:0000250\|UniProtKB:P09951}. Note=Dissociates from synaptic vesicles and redistributes into the axon during action potential firing, in a step that precedes fusion of vesicles with the plasma membrane. Reclusters to presynapses after the cessation of synaptic activity. {ECO:0000250\|UniProtKB:P09951}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.